N-terminal Sequencing

The N-terminal sequence of peptides and/or proteins can be carried out using the EDMAN degradation method on samples purified by HPLC or by SDS-PAGE (either monodimensional or bidimensional), followed by electroblotting on PVDF (PolyVinylidene DiFluoride) membranes.

Pre-treatment of the sample of the cysteine residues, is also performed, (i.e. reduction and alkylation).

The sequence of the first 10-20 residues at the N-terminal of a protein gives sufficient information to allow the following:

  • assigning the site of proteolytic maturation
  • synthesizing oligonucleotide probes for specific cDNA isolation
  • identifying the protein in databases
  • aligning the protein against DNA sequences
  • assessing the purity of the peptide/protein

Primm can also provide:

1. Preparation of samples for N-terminal sequencing 
Separation of protein samples by SDS-PAGE and blotting onto PVDF membrane.

2. Separation of peptides for internal N-terminal sequencing 
Purification of protein samples by HPLC or SDS-PAGE, enzymatic hydrolysis, fractionation of peptide mixtures, MALDIMS analysis of selected fractions.
Homogeneous peptides are submitted to automated Edman degradation

Important for sample preparation 
See technical info (PDF General information – Guide Lines for N-terminal sequencing)

  • avoid the presence of primary or secondary amine (es. do not use Tris buffer)
  • avoid high concentration of detergents and glycerol or non volatile salts
  • avoid Tris-Glycine buffer during blotting of the samples
  • use ONLY PVDF membrane for blotting procedures

For more information or a custom service quote, please fill out this short form

Your Name (required)

Your Company / Institution (required)

Your Email (required)

Your Phone

Your Message

Verification code: captcha

New England BioGroup

PO Box 1231 Atkinson,
NH 03811-1231.
Toll Free USA: 800-779-1016
Main: 617-286-4632
Fax: 617-344-0055
E-mail: info@nebiogroup.com Social: