Radio HPLC Detectors
WHAT IS RADIOCHROMATOGRAPHY?
Chromatography is a laboratory method performed to separate the different components of a mixture. The mixture is dissolved in a fluid called the mobile phase. The mobile phase carries the mixture through a column which is the stationary phase. The various compounds of the mixture travel at different speeds through the column causing them to separate.
In order to measure the separation of the mixture the compounds must be detectable in some way. There is no universal detector that can monitor all compounds, and therefore different types of HPLC detectors must be used depending on the properties of the substances to be separated. UV detectors are often used, as many substances typically separated in HPLC absorb light in the UV range of light. Other detectors such as, refractive index, light scattering, fluorescence, conductivity, and more, are also used in HPLC. The intensity of the signal measured will be represented in the graph called chromatogram. Assuming complete separation, each compound will appear in the chromatogram as a peak. The time of appearance identifies the compound, and the area of the peak quantifies it. In the case of Radio-HPLC (or radio chromatography), the substances to be separated are radioactive because they have been labeled using a radioactive isotope, such as 125I, 14C or 3H.
APPLICATIONS OF RADIO-HPLC
Radio-HPLC has numerous applications in research, diagnostics, and quality control. Some of the most common and important applications are:
Pharmaceutical Analysis: Radio-HPLC is a key technology for the separation and quantitation of radiolabeled drugs and putative metabolites in drug development.
Environmental Analysis: A wide variety of contaminants from sources such as industrial waste, landfill sites, pesticides and pharmaceutical drugs can make their way into the environment. The challenging identification of these contaminants require a highly sensitive detector to be able to detect the lowest concentrations of sample.
Development and QC of Radio chemicals: Radiochemicals are only as good as the care taken in each preparation step and strict regulations must be met during production. Radiochemical purity analysis using Radio HPLC is often required to verify the quality of the produced radiochemicals.
QC of Radiolabeled Antibodies and Proteins: Radiochemical identity and purity analysis of radiolabeled antibodies and proteins using Radio-HPLC is a simple and reliable method to ensure that only the radiolabeled antibody or protein is present in the quality control sample. 
HERM LB500 with Nal detector for gamma and high energy beta isotopes
The HERM is a dedicated radio HPLC monitor which can be connected to different detectors providing flexibility, reproducibility and sensitivity. The HERM offers a safe and user-friendly solution for all quality control laboratories. The HERM with NaI detector for gamma and high energy beta isotopes combines an extremely sensitive NaI detector with compact shielding and can detect even low energy gamma isotopes like 125I with high efficiency. For PET and direct luminescence, the HERM with fLumo detector is available.
Simple integration into every HPLC system
The HERM makes integration into any existing HPLC system very easy. The PC connection can be established instantly via the USB port or by using the analog 0-1 V output. Its compact design makes it easy to fit in to a hot cell.
Sensitive and reliable measurement
The NaI detector option combines an extremely sensitive detector with a compact shielding. Even low energy gamma isotopes like 125I can be detected with high efficiency. Lead shielding ensures low backgrounds even when there is high external radiation.
Easy operation and high flexibility
The HERM LB 500 has been designed to be intuitive and easy to use. This will get you up and running as quickly as possible. Standard tubing is used to create the flow cell volume and provides maximum flexibility. Changing cells is very easy because of the twin cell changer that enables the user to easily switch between two different cell volumes. If required by GLP regulations, the cell volume can be fixed to just one volume using the optional single volume insert. The system has been designed to avoid accidental parameter modifications eliminating manual adjustment as all instrument parameters are controlled remotely.
Dual operation mode
The HERM system can be installed in multiple distinct configurations, providing more flexibility and convenience. The comfortable stand-alone configuration provides an analog signal which can be easily integrated into an existing HPLC system. The system is equipped with a flash memory and a powerful CPU. Alternatively, the system can be installed in the PC-controlled configuration via a USB connection for digital data acquisition.
Easy-to-use software for maximum productivity
The HERM Controller is the software interface to the instrument; it controls all functions and collects the data. The RadioStar software combines the ability to run sophisticated measurements with a user-friendly interface. A Chromeleon™ driver is available, Chromeleon™ is known as a very versatile chromatography software package supporting many different hardware solutions. The driver is fully certified and digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x.
Specifications:
Detection unit: State-of-the-art ultrasensitive PMT coupled 1 inch NaI detector
Interface: USB port (mini USB connector)
Outputs: TTL signal output (raw data), analog signal 0-1 V with 16-bit resolution, 2 programmable relay outputs
Software: The following options are available: 1. External control and evaluation via RadioStar software or 2. External control and evaluation via Chromeleon™ driver.
Power Supply: 6 V DC (via wide range mains adapter)
Dimensions (W x D x H): Electronics: 210 x 60 x 120 mm, NaI detector: 140 x 180 x 100 mm, Lead shielding: 150 x 350 x 180 mm
Weight: Electronics: 1 kg, NaI detector: 2 kg, Lead shielding: 25 kg.
HERM LB500 with fLumo detector for gamma and high energy beta isotopes
The HERM is a dedicated radio HPLC monitor which can be connected to different detectors providing flexibility, reproducibility and sensitivity. The HERM with fLumo Chemiluminescence detector for HPLC systems uses a highly sensitive head-on photon counting detector with low background which is perfect for PET quality control, among other applications. Different measuring cells can be used to adapt for flow rates, activities and isotopes. For PET and direct luminescence, the HERM with Nal detector is available.
Simple integration into every HPLC system
The HERM makes integration into any existing HPLC system very easy. The PC connection can be established instantly via the USB port or by using the analog 0-1 V output. Its compact design makes it easy to fit in to a hot cell.
Highly sensitive luminescence monitoring
In the fLumo detector the low noise head-on PMT has been designed to face the sample coil directly. The result is a high sensitivity detector for even the faintest traces of chemiluminescence signals.
Background-free Gamma and PET monitoring
The use of specific measuring cells simplifies the measurement of high activity PET isotopes even in high background laboratories.
A bulky and heavy lead shielding is not required as the system is insensitive to 511 keV gamma. Available cells include:
- PET measuring cells
- Luminescence measuring cells
- Gamma measuring cells
Dual operation mode
The HERM system can be installed in multiple distinct configurations, providing more flexibility and convenience. The comfortable stand-alone configuration provides an analog signal which can be easily integrated into an existing HPLC system. The system is equipped with a flash memory and a powerful CPU. Alternatively, the system can be installed in the PC-controlled configuration via a USB connection for digital data acquisition.
Easy-to-use software for maximum productivity
The HERM Controller is the software interface to the instrument; it controls all functions and collects the data. The RadioStar software combines the ability to run sophisticated measurements with a user-friendly interface. A Chromeleon™ driver is available, Chromeleon™ is known as a very versatile chromatography software package supporting many different hardware solutions. The driver is fully certified and digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x.
Specifications:
Detection unit: Low noise photon counting 1 inch PMT
Interface: USB port (mini USB connector)
Inputs: Start signal (contact closure)
Outputs: TTL signal output (raw data), analog signal 0-1 V with 16-bit resolution, 2 programmable relay outputs
Software: The following options are available: 1. External control and evaluation via RadioStar software or 2. External control and evaluation via Chromeleon™ driver.
Power Supply: 6 V DC (via wide range mains adapter)
Dimensions (W x D x H): Electronics: 210 x 60 x 120 mm, fLumo detector: 225 x 150 x 130 mm
Weight: Electronics: 1 kg, fLumo detector: 4 kg
Maximum Flexibility, Outstanding Sensitivity
In the FlowStar² LB 514 ultimate sensitivity has been combined with touch-screen technology for quick and easy operation. Designed for maximum ease of use, safety and flexibility, the FlowStar² LB 514 sets the new standard for what a radio-HPLC flow detector should be. With over 50 years of experience Berthold Technologies is taking radio-HPLC flow detection to the next level. A wide range of available cells ensures optimal performance and supports full compatibility with both, routine and challenging applications. The convenient dual mode capability enables simple installation in different configurations and makes integration into existing systems easy.
User friendly
The FlowStar² LB 514 is designed to be intuitive and easy to use, to get you up and running quickly. The intuitive touch screen interface makes it easy to get started and keep going. Results are displayed in both, a graphical chromatogram and a numerical format, so you can start interpreting your results in real time while the run is still in progress.
The FlowStar² frees you from configuring parameters manually, thanks to the use of SmartCells: all measuring cells are equipped with a chip to automatically set the correct cell-specific system parameters. In addition, the software includes an extensive isotope library with predefined energy windows and cell usage options (14C, 3H, 32P, 33P, 35S, 128I, 111In, 99mTc…).
Easy integration into your HPLC system
The FlowStar² LB 514 makes integration easy so that everything works together to ensure consistent and reliable results. The integrated A/D converter records signals from external instruments (e.g. UV-detector) without requiring expensive additional hardware, while the USB port enables external control using RadioStar or other HPLC control software (e.g. Chromeleon™).
In addition, the dual analog output allows for the convenient integration into existing HPLC data systems.
The FlowStar² supports UHPLC and other LC technologies:
- UHPLC
- HPLC-MS (Mass Spectrometry)
- HPLC-NMR (Nuclear Magnetic Resonance)
Safe and secure
The FlowStar² LB 514 embodies our commitment to providing everything you need for safe and easy integration into your lab environment.
Safety features:
- Automatic HV shutdown for system safety.
- Built-in leak detection to prevent the measuring system from damage caused by aggressive liquids. The system will shut down automatically in the event of cell leakage.
Security features:
- On-board system performance tests with instrument-based history function.
- Password-based multi user access control to protect instrument from unauthorised use.
Validation:
Several test cells are available to perform periodic system performance tests. These cells have been developed to meet GLP/GMP requirements and to guarantee a continuous system performance. Contact us for more information.
High sensitivity
The FlowStar² is the ultimate platform for high-sensitivity radio-HPLC flow detection. It uses random coincidence counting and luminescence subtraction for ultimate performance. In addition, the integrated spectrum scanning feature provides improved optimization of detector setup for each isotope.
Easy-to-use software for maximum productivity
The RadioStar software combines the ability to run sophisticated measurements with a user-friendly operation and user interface. In addition, a Chromeleon™ driver is available. Chromeleon™ is known as a very versatile chromatograpy software package supporting many different hardware solutions. The driver is fully certified, digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x
Measuring cells specific to your needs
Choose from a wide range of available SmartCells to meet your application needs. These high sensitivity cells are equipped with a SmartChip to automatically set the correct cell-specific system parameters. The automatic shut-down feature of the system after removal of a cell from the detector provides additional safety.
The range of SmartCells include:
- Liquid scintillation (admixture) cells
- Solid scintillator cells
- Gamma isotopes and Positron Emitter (PET) cells
- Cells for preparative SFC
- Microbore cells for UHPLC and UPLC™
- Special Iodine Cells (125I)
- Cherenkov cells for high energy beta emitters
Specifications
Display: Graphical touch screen with 320 x 240 pixel resolution.
Interface: USB port (B-type), RS-232 for pump control
Inputs: Start-, Stop- and Ready signal (TTL). 2 analog inputs (24 bit resolution), variable voltage (bipolar, max 2.5 V)
Outputs: Scintillator pump control (RS-232 and analog). 2 analog signal outputs 0-1 V (2,5 times oversampling) with 16-bit resolution. Waste valve and fraction collector control output (open collector output).
Power Supply: 90-264 V, 50/60 Hz
Dimensions (W x D x H): 410 x 170 x 410 mm. Horizontal and vertical operation possible.
Weight: 16kg
Operating Temperature: 10-35°C
Storage Temperature: 5-40°C
Humidity: 10-90% (non condensing)
Software: The following possibilities are available:
- Built-in software operated with touch screen
- External control and evaluation via RadioStar software
- External control and evaluation via Chromeleon™ driver.
New England BioGroup
NH 03811-1231.
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Main: 617-286-4632
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