Automation
Download the Cellometer Auto T4 brochure
Cellometer AutoT4 is a compact, simple to use, automated cell counter designed to improve accuracy, repeatability, and throughput compared to manual cell counting with the hemacytometer. It uses brightfield microscopy images to analyze cell samples to determine cell concentration and trypan blue viability.
Best Suited For:
- total cell concentration for highly viable cell population
- live cell concentration and viability using trypan blue
- cell size measurements
- identify and count cells based on cell size difference
- capture and store sell images
Fast, Accurate Automated Cell Counter
Ideal For Determining Concentration And Trypan Blue Viability And Cell Lines
Minimal Sample Prep
Disposable counting chambers is the only consumable needed to run Cellometer AutoT4. Only 20 µL of sample is required, in the chambers load in seconds, and when done can be disposed, eliminating the need for coverslips and washing between samples.
Trypan Blue Viability
Trypan blue treated cells are automatically counted as live or dead, and the percentage viability is automatically calculated. Live counted cells are indicated by a green circle on screen, and dead cells by a red circle.
On-screen Imaging
No two cells are the same, which is why live cell morphology can be immediately viewed on screen, and counted cells are indicated on screen for further analysis. Accounting results are indicated for each cell in images can be saved or exported for a secure record of all your cell counts.
Fast! From loading counting chamber to view cell morphology and counting, the Cellometetr Auto T4 typically produces the cell concentration and viability in less than 30 seconds!
Exclude Cellular Debris
Because the Cellometer AutoT4 recognizes cells based on size, brightness, and morphology, cellular debris can be excluded from counting results, ensuring accurate data.
Heterogeneous Cell Samples
Minimum and maximum cell size parameters can be set to only count specific cells in a heterogeneous sample. This is ideal for counting dendritic cells cultured from PBMC, or counting PBMC's amongst platelets.
Irregular Cell Shapes
The Cellometer AutoT4 can recognize and count irregular shaped cells by adjusting the roundness cell setting. This feature is ideal for counting RD cells were activated T-cells
Cell Size Measurement
Unlike manual counting, the Cellometer AutoT4 measures both individual and mean cell sizes, and can generate size distribution histograms for all counted cells. Multiple histograms can be overlaid for determining changes in cell sizes over time.
Changes in Cell Diameter
Because the Cellometer AutoT4 generates individual cell size measurements, multiple samples can be overlaid on one histogram, enabling analysis of change in cell diameter over time, such as when counting SF9 or SF21 cells. Saved images can be viewed and counted later to analyze changes in morphology over time.
MOVE BEYOND CELL COUNTING TO TRUE INFORMATION SYSTEMS!
With a single mouse click, you can save the sample image with data to disk, print the results, display cell size distribution and a histogram, or populate an Excel spreadsheet for additional analysis.
http://youtu.be/tN-Z-vF68xs
Cellometer AutoX4 is a line of simple to use, automated cell counters with fluorescence detection. It combines brightfield microscopy with fluorescence to identify selected cells. The user can switch fluorescence optics modules to detect a wide range of fluorophores with excitation wavelengths from UV to deep red.
Best suited for:
- Total cell concentration for highly viable cell concentration
- Live cell concentration and viability using Trypan blue
- Cell size measurement
- Identify and count cells based on cell size difference
- Capture and store cell images
- Total nucleated cell concentration without lysing non-nucleated cells
- Live nucleated cell concentration without lysing non-nucleated cells
- Fluorescence based live cell concentration and viability for cell lines
- AO, PI, EB, DAPI, Hoechst
In addition to what is available with the Cellometer Auto line of cell counters, Cellometer Auto X4 has the following enhanced functionalities and capabilities:
- Brightfield (BR) and fluorescent(FL) image capabilities
- Brightfield assays for total cell count or trypan blue viability
- Fluorescence assays of DNA staining dye based cell counting for nucleated cells
- Brightfield and fluorescence combination assays for DNA staining dye or Esterase substrat based viability assays
- User switchable fluorescence optics modules for different dyes
- Software pre-installed for most common assays
View the Cellometer Auto 2000 brochure
Automatic cell viability counter with dual fluorescence. Consistent, reliable, and accurate for primary cells.
Cellometer Auto 2000 Standard Assays
Cellometer Vision is a line of simple to use, automated cell counters and cell analyzers with diverse functions. It combines brightfield microscopy and multi-channel fluorescence images to generate cell count and fluorescence data. It is most suitable for determining cell concentration and viability in complex samples. The sophisticated data analysis software allows detailed studies of select cell population.
Cellometer Vision is the Cell Counter Best Suited For:
- Total cell concentration for highly viable cell population
- Counting primary hepatocyte cells
- Live cell concentration and viability using trypan blue
- Cell size measurement
- Identify and count cells based on cell size difference
- Capture and store cell images
- Total nucleated cell concentration without lysing non-nucleated cells
- Live nucleated cell concentration without lysing non-nucleated cells
- Fluorescence based live cell concentration and viability for cell lines
- Transfection efficiency by GFP
- Transfection efficiency by YFP
- Transfection efficiency by RFP
- Live cell concentration and viability using two fluorescents in one sample
- AO, PI, EB, DAPI, Hoechst
1) Pipette 20µL Sample
2) Insert Slide
3) Get Data
Whether you need to determine rates of GFP transfection, are involved in primary hepatocyte isolation and need to count primary hepatocytes from mouse, human, rat or other types of samples, the Cellometer Vision automated cell counter can offer you some value. Contact us to learn more about this instrument or to have us visit the lab for a demonstration. We work closely with our customers and work to place a cell counter in the lab that best fits your needs.
New England BioGroup
NH 03811-1231.
Toll Free USA: 800-779-1016
Main: 617-286-4632
Fax: 617-344-0055
E-mail: info@nebiogroup.com Social:
